05);此外,鞘内注射MRS2211并不影响CCI大鼠背角P2X4受体表达变化。结论:鞘内注射P2Y13受体拮抗剂MRS2211可以明显抑制CCI大鼠热痛敏症状和脊髓背角P38MAPK表达的上调,但并不影响CCI大鼠P2X4受体表达变化。这提示MRS2211可以抑制小胶质细胞P38MAPK活化但不影响小胶质细胞P2X4受体表达,这可能是其在脊髓水平发挥镇痛作用的机制之一。
目的观察降逆护膜汤体外对脱氧胆酸(DCA)诱导人正常食管黏膜上皮细胞HEEC凋亡中p38MAPK相关基因表达的影响,探讨降逆护膜汤治疗胃食管反流病的机制。方法通过体外培养人正常食管上皮细胞,经DCA诱导出现凋亡现象,采用MTT法观察不同浓度的降逆护膜汤对细胞凋亡的影响。运用western 而且 blot检测细胞凋亡与p38MAPK的关系。结果 MTT显示与正常对照组相比,DCA使细胞生存率明显降低(P<0.01),而降逆护膜汤提高生存率,与DCA组比较有统计学意义(P
Irisin是一种新发现的肌肉因子,由骨骼肌分泌并随运动增加,是一个包含111个氨基酸的多肽激素,由FNDC5基因的产物蛋白水解后释放入血液循环,受过氧化物酶体增生物激活受体γ辅激活子-1α(PGC-1α)调节。该因子可促进皮下脂肪的棕色化以及解耦联蛋白1(UCP1)的表达,从而增加产热。经研究证实,传统的棕色脂肪或者白色脂肪棕色化可以改善人体肥胖和葡萄糖的平衡。因此Irisin也可能
目的:探讨神经节甘酯与神经生长因子联合高压氧在颅脑损伤患者中的临床治疗效果。方法:对诊断、治疗的80例颅脑损伤患者相关资料进行分析,对照组采用神经节甘酯与神经生长因子治疗,实验组联合高压氧治疗,比较两组治疗效果。结果:实验组采用神经节甘酯与神经生长因子联合高压氧治疗后95%疗效确切,高于对照组(85%)(P<0.05);实验组意识觉醒时间(6.29±0.94)天,短于对照组(8.16±0.82)天(P<0.05);实验组NIHSS评分(10.8±1.16)分,低于对照组(12.87±0.92)分(P<0.05)。结论:采用神经节甘酯与神经生长因子联合高压氧治疗颅脑损伤效果理想,值得推广使用。
肝细胞癌是临床常见恶性肿瘤之一,早期诊断困难,手术切除率低,术后复发与转移率高,目前尚无治疗肝癌及其复发与转移的特异方法。细胞自噬是一种细胞自我消化过程,在能量缺乏、外界刺激等压力下,机体将细胞内变性、损伤、衰老或非功能性的蛋白、细胞器和细胞质包裹、消化,降解成为核苷、氨基酸和脂肪酸循环利用,实现细胞自身代谢需要和细胞器的更新,对于细胞生存、分化以及自身稳态的维持具有重要的生物学作用。肝癌细胞依靠细胞自噬作用维持生存和生物学活性,细胞自噬对肝细胞癌产生”保护”或”杀伤”不同作用,深入研究细胞自噬在肝细胞癌发生、发展中出现的双重调控作用,对于揭示肝细胞癌发生、发展机制和防治具有重要的生物学意义。
Normal

购买Afatinib mouse pluripotent stem cells were originally derived from the inner cell mass(ICM) of blastocysts and shown to be the in vitro equivalent of those pre-implantation embryonic cells, and thus were called embryonic stem cells(ESCs). More than a decade later, pluripotent cells were isolated from the ICM of human blastocysts. Despite being called human ESCs, these cells differ significantly from mouse ESCs, including different morphology and mechanisms of control of pluripotency, suggesting distinct embryonic origins of ESCs from the two species. Subsequently, mouse pluripotent stem cells were established

from the ICMderived epiblast of post-implantation embryos. These mouse epiblast stem cells(Epi SCs) are 也许 morphological and epigenetically more similar to human ESCs. This raised the question of whether cells from the human ICM are in a more advanced differentiation stage than their murine counterpart, or whether the available culture conditions were not adequate to maintain those human cells in their in vivo state, leading to a transition into Epi SC-like cells in vitro. More recently, novel culture conditions allowed the conversion of human ESCs into mouse ESC-like cells called nave(or ground state) human ESCs, and the derivation of nave human ESCs from blastocysts.